Morphometrics and the Ultrasonographic Ovarian Appearance of Sexually Mature Female Bornean Earless Monitors (Lanthanotus borneensis)
There is a lack of reproductive information regarding the endangered Bornean earless monitor (Lanthanotus borneensis), including on how to determine its sex. The objective of this study was to identify sex in a zoo population of 10 healthy Bornean earless monitors as well as define the ultrasonographic appearance of sexually mature female ovaries. Morphometrics of this population, including snout–vent length, tail length, head length, head width, head height, length of the dentary, dentary height, and lower jaw width were recorded. In addition, coelomic ultrasounds under manual restraint were performed. Total follicles per animal (7.55 ± 2.06, mean ± SD), follicular volume (0.32 ± 0.20 cm3), and type of follicles (hypoechoic, vitellogenic, and laminar) were recorded for each female in the study population. Although morphometrics were not useful in determining sex, ultrasound was a reliable, noninvasive method for this species. In addition, baseline measurements regarding their ovarian morphology were determined that may be useful for future breeding and health assessments for this endangered species.Abstract

Transverse ultrasound using the Vet IQ+ probe on the ventral coelom of a Bornean earless monitor lizard (Lanthanotus borneensis).

Ovarian images of a Bornean earless monitor lizard (Lanthanotus borneensis). (A) Hypoechoic follicle that is characteristic of a reproductively inactive female. (B) Vitellogenic follicles are shown with an increased echotexture compared with the surrounding tissue. (C) Enlarged follicles that show a laminar appearance to the follicles are often maturing to the point of ovulation.

Left ovary of a Bornean earless monitor lizard (Lanthanotus borneensis). The lines within the image represent the measurements taken of the largest follicles for data analysis.

Ultrasound showing the appearance of the testicle of a Bornean earless monitor lizard (Lanthanotus borneensis).
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