Feasibility, Gross Postmortem Results and Participant Perception of Oblique Prefemoral Ovariectomy in Red Eared Sliders (Trachemys scripta elegans) During a Teaching Workshop
The purpose of the present report is to describe feasibility and gross postmortem results of oblique prefemoral ovariectomy in red-eared sliders (Trachemys scripta elegans) performed by veterinarians in a teaching environment. Sixteen red-eared sliders were included in a teaching workshop. The turtles were anesthetized and placed in dorsolateral recumbency at an oblique 45-degree angle by use of folded towels. After prefemoral access to the celom and placement of a retractor with elastic bands, the ipsilateral follicles were exteriorized via gentle maneuvering with cotton tip applicators and excised after application of titanium clips and cautery with bipolar radiosurgery. On 14 of the 16 chelonians (87.5%) surgery was successfully completed. Of the two chelonians that did not have the surgical procedure completed, one had marked celomitis with adhesion of the ovaries to the liver and one had immature ovaries. At the end of the surgical procedures all chelonians were euthanized and postmortem was performed. The 14 chelonians that had surgery completed had no evident remaining ovarian tissue on gross postmortem. A survey completed by the attendants 9 mo after the workshop showed that a minority of the respondents (1/17) found the procedure difficult and that one of the seven respondents that performed the surgery after the workshop was not able to complete the procedure. This report indicates that oblique prefemoral ovariectomy in mature red-eared sliders can be effectively taught to veterinarians during a workshop, and that if the procedure is completed, the chances of leaving ovarian remnants are low.Abstract

Representative photograph of a red-eared slider (Trachemys scripta elegans) in a dorsolateral, oblique 45-degree angle recumbency before ovariectomy. The photograph was not obtained during the teaching workshop, but during clinical practice and is used for illustrative purposes. Notice the proximity to the edge of the surgical table and the elevation of the side far from the surgeon.

Representative photograph of the limited exteriorization of the ovary that occurred in some red-eared sliders (Trachemys scripta elegans) during the teaching workshop. While in some specimens the ovary was completely exteriorized with external visualization of the mesovarium, in other specimens the proximal part of the ovary could not be fully exteriorized and hemostasis was performed with titanium clips and bipolar cautery intracelomically.

Postmortem findings in the two red-eared sliders (Trachemys scripta elegans) (no. 2 and no. 5) that did not have a complete ovariectomy. A. Specimen no. 2 was sexually immature, with short ovaries characterized by multiple nonvitellogenic follicles tightly adherent to the stroma. B. Specimen no. 5 had marked celomitis with adhesions extending from both ovaries to the liver. Cr = cranial; Ca = caudal; L = liver; O = ovary; S = salpinx.